pgl3-enhancer vector (Promega)
90
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Promega
pgl3-enhancer vector
Pgl3 Enhancer Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3-enhancer+vector/pgl3+basic/us12006498-169-18-20
Average 90 stars, based on 1 article reviews
Pgl3 Enhancer Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3-enhancer+vector/pgl3+basic/us12006498-169-18-20
Average 90 stars, based on 1 article reviews
pgl3-enhancer vector - by Bioz Stars,
2026-10
90/100 stars
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Polymerase Chain Reaction:Article Title: SINE Insertion in the Pig Carbonic Anhydrase 5B (CA5B) Gene Is Associated with Changes in Gene Expression and Phenotypic Variation. Article Snippet: These fragments were each inserted into the Article Title: NGX6 gene mediated by promoter methylation as a potential molecular marker in colorectal cancer Article Snippet: In order to identify the proximal promoter region of NGX6 gene, four constructs of progressive deletion were generated and cloned to the upstream of the luciferase reporter gene in the Article Title: Promoter Hypermethylation-mediated Inactivation of LRRC4 in Gliomas Article Snippet: The 5' upsteam regulatory region of the LRRC4 gene was subcloned into the KpnI and Bgl II restriction sites of the Article Title: Regulation of Cysteinyl Leukotriene Receptor 2 Expression—A Potential Anti-Tumor Mechanism Article Snippet: Human CysLT 2 R promoter fragments were subcloned into a Article Title: A Genetic Variant in the IL-17 Promoter Is Functionally Associated with Acute Graft-Versus-Host Disease after Unrelated Bone Marrow Transplantation Article Snippet: The resultant fragments IL-17/197A or IL-17/197G were subcloned into the pGL3-enhancer vector at the MluI and Article Title: Genetic Variants in Telomerase Reverse Transcriptase Contribute to Solar Lentigines. Article Snippet: Plasmid construction and luciferase assays Telomerase reverse transcriptase (TERT) promoter region was isolated from human genomic DNA using PCR, and the PCR products were cut with KpnI and XhoI or XhoI and HindIII restriction enzymes (New England Biolabs, Ipswich, MA), gel purified, and subcloned into pGL3-Enhancer Vector or pGL3Promoter Vector (Promega, Madison, WI). Article Title: Composition for regulating expression of PROX1 or expression of telomerase reverse transcriptase comprising activity regulator or method for screening telomerase reverse transcriptase regulator Article Snippet: The PCR product was digested with SacI and XhoI, and the prepared amplicons 1-3 were inserted into a Article Title: Egr-1 regulates the transcription of NGX6 gene through a Sp1/Egr-1 overlapping site in the promoter Article Snippet: These deletion fragments were cleaved, gel-purified and cloned into Plasmid Preparation:Article Title: SINE Insertion in the Pig Carbonic Anhydrase 5B (CA5B) Gene Is Associated with Changes in Gene Expression and Phenotypic Variation. Article Snippet: These fragments were each inserted into the Article Title: NGX6 gene mediated by promoter methylation as a potential molecular marker in colorectal cancer Article Snippet: In order to identify the proximal promoter region of NGX6 gene, four constructs of progressive deletion were generated and cloned to the upstream of the luciferase reporter gene in the Article Title: Promoter Hypermethylation-mediated Inactivation of LRRC4 in Gliomas Article Snippet: The 5' upsteam regulatory region of the LRRC4 gene was subcloned into the KpnI and Bgl II restriction sites of the Article Title: Regulation of Cysteinyl Leukotriene Receptor 2 Expression—A Potential Anti-Tumor Mechanism Article Snippet: Human CysLT 2 R promoter fragments were subcloned into a Article Title: A Genetic Variant in the IL-17 Promoter Is Functionally Associated with Acute Graft-Versus-Host Disease after Unrelated Bone Marrow Transplantation Article Snippet: The resultant fragments IL-17/197A or IL-17/197G were subcloned into the pGL3-enhancer vector at the MluI and Article Title: Genetic Variants in Telomerase Reverse Transcriptase Contribute to Solar Lentigines. Article Snippet: Plasmid construction and luciferase assays Telomerase reverse transcriptase (TERT) promoter region was isolated from human genomic DNA using PCR, and the PCR products were cut with KpnI and XhoI or XhoI and HindIII restriction enzymes (New England Biolabs, Ipswich, MA), gel purified, and subcloned into pGL3-Enhancer Vector or pGL3Promoter Vector (Promega, Madison, WI). Article Title: Composition for regulating expression of PROX1 or expression of telomerase reverse transcriptase comprising activity regulator or method for screening telomerase reverse transcriptase regulator Article Snippet: The PCR product was digested with SacI and XhoI, and the prepared amplicons 1-3 were inserted into a Article Title: Egr-1 regulates the transcription of NGX6 gene through a Sp1/Egr-1 overlapping site in the promoter Article Snippet: These deletion fragments were cleaved, gel-purified and cloned into Construct:Article Title: SINE Insertion in the Pig Carbonic Anhydrase 5B (CA5B) Gene Is Associated with Changes in Gene Expression and Phenotypic Variation. Article Snippet: These fragments were each inserted into the Article Title: NGX6 gene mediated by promoter methylation as a potential molecular marker in colorectal cancer Article Snippet: In order to identify the proximal promoter region of NGX6 gene, four constructs of progressive deletion were generated and cloned to the upstream of the luciferase reporter gene in the Article Title: Promoter Hypermethylation-mediated Inactivation of LRRC4 in Gliomas Article Snippet: The 5' upsteam regulatory region of the LRRC4 gene was subcloned into the KpnI and Bgl II restriction sites of the Article Title: Regulation of Cysteinyl Leukotriene Receptor 2 Expression—A Potential Anti-Tumor Mechanism Article Snippet: Human CysLT 2 R promoter fragments were subcloned into a Article Title: A Genetic Variant in the IL-17 Promoter Is Functionally Associated with Acute Graft-Versus-Host Disease after Unrelated Bone Marrow Transplantation Article Snippet: The resultant fragments IL-17/197A or IL-17/197G were subcloned into the pGL3-enhancer vector at the MluI and Article Title: Genetic Variants in Telomerase Reverse Transcriptase Contribute to Solar Lentigines. Article Snippet: Plasmid construction and luciferase assays Telomerase reverse transcriptase (TERT) promoter region was isolated from human genomic DNA using PCR, and the PCR products were cut with KpnI and XhoI or XhoI and HindIII restriction enzymes (New England Biolabs, Ipswich, MA), gel purified, and subcloned into pGL3-Enhancer Vector or pGL3Promoter Vector (Promega, Madison, WI). Article Title: Composition for regulating expression of PROX1 or expression of telomerase reverse transcriptase comprising activity regulator or method for screening telomerase reverse transcriptase regulator Article Snippet: The PCR product was digested with SacI and XhoI, and the prepared amplicons 1-3 were inserted into a Article Title: Egr-1 regulates the transcription of NGX6 gene through a Sp1/Egr-1 overlapping site in the promoter Article Snippet: These deletion fragments were cleaved, gel-purified and cloned into Luciferase:Article Title: SINE Insertion in the Pig Carbonic Anhydrase 5B (CA5B) Gene Is Associated with Changes in Gene Expression and Phenotypic Variation. Article Snippet: These fragments were each inserted into the Article Title: NGX6 gene mediated by promoter methylation as a potential molecular marker in colorectal cancer Article Snippet: In order to identify the proximal promoter region of NGX6 gene, four constructs of progressive deletion were generated and cloned to the upstream of the luciferase reporter gene in the Article Title: Promoter Hypermethylation-mediated Inactivation of LRRC4 in Gliomas Article Snippet: The 5' upsteam regulatory region of the LRRC4 gene was subcloned into the KpnI and Bgl II restriction sites of the Article Title: Regulation of Cysteinyl Leukotriene Receptor 2 Expression—A Potential Anti-Tumor Mechanism Article Snippet: Human CysLT 2 R promoter fragments were subcloned into a Article Title: A Genetic Variant in the IL-17 Promoter Is Functionally Associated with Acute Graft-Versus-Host Disease after Unrelated Bone Marrow Transplantation Article Snippet: The resultant fragments IL-17/197A or IL-17/197G were subcloned into the pGL3-enhancer vector at the MluI and Article Title: Genetic Variants in Telomerase Reverse Transcriptase Contribute to Solar Lentigines. Article Snippet: Plasmid construction and luciferase assays Telomerase reverse transcriptase (TERT) promoter region was isolated from human genomic DNA using PCR, and the PCR products were cut with KpnI and XhoI or XhoI and HindIII restriction enzymes (New England Biolabs, Ipswich, MA), gel purified, and subcloned into pGL3-Enhancer Vector or pGL3Promoter Vector (Promega, Madison, WI). Article Title: Composition for regulating expression of PROX1 or expression of telomerase reverse transcriptase comprising activity regulator or method for screening telomerase reverse transcriptase regulator Article Snippet: The PCR product was digested with SacI and XhoI, and the prepared amplicons 1-3 were inserted into a Article Title: Egr-1 regulates the transcription of NGX6 gene through a Sp1/Egr-1 overlapping site in the promoter Article Snippet: These deletion fragments were cleaved, gel-purified and cloned into Isolation:Article Title: SINE Insertion in the Pig Carbonic Anhydrase 5B (CA5B) Gene Is Associated with Changes in Gene Expression and Phenotypic Variation. Article Snippet: These fragments were each inserted into the Article Title: NGX6 gene mediated by promoter methylation as a potential molecular marker in colorectal cancer Article Snippet: In order to identify the proximal promoter region of NGX6 gene, four constructs of progressive deletion were generated and cloned to the upstream of the luciferase reporter gene in the Article Title: Promoter Hypermethylation-mediated Inactivation of LRRC4 in Gliomas Article Snippet: The 5' upsteam regulatory region of the LRRC4 gene was subcloned into the KpnI and Bgl II restriction sites of the Article Title: Regulation of Cysteinyl Leukotriene Receptor 2 Expression—A Potential Anti-Tumor Mechanism Article Snippet: Human CysLT 2 R promoter fragments were subcloned into a Article Title: A Genetic Variant in the IL-17 Promoter Is Functionally Associated with Acute Graft-Versus-Host Disease after Unrelated Bone Marrow Transplantation Article Snippet: The resultant fragments IL-17/197A or IL-17/197G were subcloned into the pGL3-enhancer vector at the MluI and Article Title: Genetic Variants in Telomerase Reverse Transcriptase Contribute to Solar Lentigines. Article Snippet: Plasmid construction and luciferase assays Telomerase reverse transcriptase (TERT) promoter region was isolated from human genomic DNA using PCR, and the PCR products were cut with KpnI and XhoI or XhoI and HindIII restriction enzymes (New England Biolabs, Ipswich, MA), gel purified, and subcloned into pGL3-Enhancer Vector or pGL3Promoter Vector (Promega, Madison, WI). Article Title: Composition for regulating expression of PROX1 or expression of telomerase reverse transcriptase comprising activity regulator or method for screening telomerase reverse transcriptase regulator Article Snippet: The PCR product was digested with SacI and XhoI, and the prepared amplicons 1-3 were inserted into a Article Title: Egr-1 regulates the transcription of NGX6 gene through a Sp1/Egr-1 overlapping site in the promoter Article Snippet: These deletion fragments were cleaved, gel-purified and cloned into Purification:Article Title: SINE Insertion in the Pig Carbonic Anhydrase 5B (CA5B) Gene Is Associated with Changes in Gene Expression and Phenotypic Variation. Article Snippet: These fragments were each inserted into the Article Title: NGX6 gene mediated by promoter methylation as a potential molecular marker in colorectal cancer Article Snippet: In order to identify the proximal promoter region of NGX6 gene, four constructs of progressive deletion were generated and cloned to the upstream of the luciferase reporter gene in the Article Title: Promoter Hypermethylation-mediated Inactivation of LRRC4 in Gliomas Article Snippet: The 5' upsteam regulatory region of the LRRC4 gene was subcloned into the KpnI and Bgl II restriction sites of the Article Title: Regulation of Cysteinyl Leukotriene Receptor 2 Expression—A Potential Anti-Tumor Mechanism Article Snippet: Human CysLT 2 R promoter fragments were subcloned into a Article Title: A Genetic Variant in the IL-17 Promoter Is Functionally Associated with Acute Graft-Versus-Host Disease after Unrelated Bone Marrow Transplantation Article Snippet: The resultant fragments IL-17/197A or IL-17/197G were subcloned into the pGL3-enhancer vector at the MluI and Article Title: Genetic Variants in Telomerase Reverse Transcriptase Contribute to Solar Lentigines. Article Snippet: Plasmid construction and luciferase assays Telomerase reverse transcriptase (TERT) promoter region was isolated from human genomic DNA using PCR, and the PCR products were cut with KpnI and XhoI or XhoI and HindIII restriction enzymes (New England Biolabs, Ipswich, MA), gel purified, and subcloned into pGL3-Enhancer Vector or pGL3Promoter Vector (Promega, Madison, WI). Article Title: Composition for regulating expression of PROX1 or expression of telomerase reverse transcriptase comprising activity regulator or method for screening telomerase reverse transcriptase regulator Article Snippet: The PCR product was digested with SacI and XhoI, and the prepared amplicons 1-3 were inserted into a Article Title: Egr-1 regulates the transcription of NGX6 gene through a Sp1/Egr-1 overlapping site in the promoter Article Snippet: These deletion fragments were cleaved, gel-purified and cloned into Transfection:Article Title: SINE Insertion in the Pig Carbonic Anhydrase 5B (CA5B) Gene Is Associated with Changes in Gene Expression and Phenotypic Variation. Article Snippet: These fragments were each inserted into the Article Title: NGX6 gene mediated by promoter methylation as a potential molecular marker in colorectal cancer Article Snippet: In order to identify the proximal promoter region of NGX6 gene, four constructs of progressive deletion were generated and cloned to the upstream of the luciferase reporter gene in the Article Title: Promoter Hypermethylation-mediated Inactivation of LRRC4 in Gliomas Article Snippet: The 5' upsteam regulatory region of the LRRC4 gene was subcloned into the KpnI and Bgl II restriction sites of the Article Title: Regulation of Cysteinyl Leukotriene Receptor 2 Expression—A Potential Anti-Tumor Mechanism Article Snippet: Human CysLT 2 R promoter fragments were subcloned into a Article Title: A Genetic Variant in the IL-17 Promoter Is Functionally Associated with Acute Graft-Versus-Host Disease after Unrelated Bone Marrow Transplantation Article Snippet: The resultant fragments IL-17/197A or IL-17/197G were subcloned into the pGL3-enhancer vector at the MluI and Article Title: Genetic Variants in Telomerase Reverse Transcriptase Contribute to Solar Lentigines. Article Snippet: Plasmid construction and luciferase assays Telomerase reverse transcriptase (TERT) promoter region was isolated from human genomic DNA using PCR, and the PCR products were cut with KpnI and XhoI or XhoI and HindIII restriction enzymes (New England Biolabs, Ipswich, MA), gel purified, and subcloned into pGL3-Enhancer Vector or pGL3Promoter Vector (Promega, Madison, WI). Article Title: Composition for regulating expression of PROX1 or expression of telomerase reverse transcriptase comprising activity regulator or method for screening telomerase reverse transcriptase regulator Article Snippet: The PCR product was digested with SacI and XhoI, and the prepared amplicons 1-3 were inserted into a Article Title: Egr-1 regulates the transcription of NGX6 gene through a Sp1/Egr-1 overlapping site in the promoter Article Snippet: These deletion fragments were cleaved, gel-purified and cloned into Expressing:Article Title: SINE Insertion in the Pig Carbonic Anhydrase 5B (CA5B) Gene Is Associated with Changes in Gene Expression and Phenotypic Variation. Article Snippet: These fragments were each inserted into the Article Title: NGX6 gene mediated by promoter methylation as a potential molecular marker in colorectal cancer Article Snippet: In order to identify the proximal promoter region of NGX6 gene, four constructs of progressive deletion were generated and cloned to the upstream of the luciferase reporter gene in the Article Title: Promoter Hypermethylation-mediated Inactivation of LRRC4 in Gliomas Article Snippet: The 5' upsteam regulatory region of the LRRC4 gene was subcloned into the KpnI and Bgl II restriction sites of the Article Title: Regulation of Cysteinyl Leukotriene Receptor 2 Expression—A Potential Anti-Tumor Mechanism Article Snippet: Human CysLT 2 R promoter fragments were subcloned into a Article Title: A Genetic Variant in the IL-17 Promoter Is Functionally Associated with Acute Graft-Versus-Host Disease after Unrelated Bone Marrow Transplantation Article Snippet: The resultant fragments IL-17/197A or IL-17/197G were subcloned into the pGL3-enhancer vector at the MluI and Article Title: Genetic Variants in Telomerase Reverse Transcriptase Contribute to Solar Lentigines. Article Snippet: Plasmid construction and luciferase assays Telomerase reverse transcriptase (TERT) promoter region was isolated from human genomic DNA using PCR, and the PCR products were cut with KpnI and XhoI or XhoI and HindIII restriction enzymes (New England Biolabs, Ipswich, MA), gel purified, and subcloned into pGL3-Enhancer Vector or pGL3Promoter Vector (Promega, Madison, WI). Article Title: Composition for regulating expression of PROX1 or expression of telomerase reverse transcriptase comprising activity regulator or method for screening telomerase reverse transcriptase regulator Article Snippet: The PCR product was digested with SacI and XhoI, and the prepared amplicons 1-3 were inserted into a Article Title: Egr-1 regulates the transcription of NGX6 gene through a Sp1/Egr-1 overlapping site in the promoter Article Snippet: These deletion fragments were cleaved, gel-purified and cloned into Cell Culture:Article Title: SINE Insertion in the Pig Carbonic Anhydrase 5B (CA5B) Gene Is Associated with Changes in Gene Expression and Phenotypic Variation. Article Snippet: These fragments were each inserted into the Article Title: NGX6 gene mediated by promoter methylation as a potential molecular marker in colorectal cancer Article Snippet: In order to identify the proximal promoter region of NGX6 gene, four constructs of progressive deletion were generated and cloned to the upstream of the luciferase reporter gene in the Article Title: Promoter Hypermethylation-mediated Inactivation of LRRC4 in Gliomas Article Snippet: The 5' upsteam regulatory region of the LRRC4 gene was subcloned into the KpnI and Bgl II restriction sites of the Article Title: Regulation of Cysteinyl Leukotriene Receptor 2 Expression—A Potential Anti-Tumor Mechanism Article Snippet: Human CysLT 2 R promoter fragments were subcloned into a Article Title: A Genetic Variant in the IL-17 Promoter Is Functionally Associated with Acute Graft-Versus-Host Disease after Unrelated Bone Marrow Transplantation Article Snippet: The resultant fragments IL-17/197A or IL-17/197G were subcloned into the pGL3-enhancer vector at the MluI and Article Title: Genetic Variants in Telomerase Reverse Transcriptase Contribute to Solar Lentigines. Article Snippet: Plasmid construction and luciferase assays Telomerase reverse transcriptase (TERT) promoter region was isolated from human genomic DNA using PCR, and the PCR products were cut with KpnI and XhoI or XhoI and HindIII restriction enzymes (New England Biolabs, Ipswich, MA), gel purified, and subcloned into pGL3-Enhancer Vector or pGL3Promoter Vector (Promega, Madison, WI). Article Title: Composition for regulating expression of PROX1 or expression of telomerase reverse transcriptase comprising activity regulator or method for screening telomerase reverse transcriptase regulator Article Snippet: The PCR product was digested with SacI and XhoI, and the prepared amplicons 1-3 were inserted into a Article Title: Egr-1 regulates the transcription of NGX6 gene through a Sp1/Egr-1 overlapping site in the promoter Article Snippet: These deletion fragments were cleaved, gel-purified and cloned into Clone Assay:Article Title: SINE Insertion in the Pig Carbonic Anhydrase 5B (CA5B) Gene Is Associated with Changes in Gene Expression and Phenotypic Variation. Article Snippet: These fragments were each inserted into the Article Title: NGX6 gene mediated by promoter methylation as a potential molecular marker in colorectal cancer Article Snippet: In order to identify the proximal promoter region of NGX6 gene, four constructs of progressive deletion were generated and cloned to the upstream of the luciferase reporter gene in the Article Title: Promoter Hypermethylation-mediated Inactivation of LRRC4 in Gliomas Article Snippet: The 5' upsteam regulatory region of the LRRC4 gene was subcloned into the KpnI and Bgl II restriction sites of the Article Title: Regulation of Cysteinyl Leukotriene Receptor 2 Expression—A Potential Anti-Tumor Mechanism Article Snippet: Human CysLT 2 R promoter fragments were subcloned into a Article Title: A Genetic Variant in the IL-17 Promoter Is Functionally Associated with Acute Graft-Versus-Host Disease after Unrelated Bone Marrow Transplantation Article Snippet: The resultant fragments IL-17/197A or IL-17/197G were subcloned into the pGL3-enhancer vector at the MluI and Article Title: Genetic Variants in Telomerase Reverse Transcriptase Contribute to Solar Lentigines. Article Snippet: Plasmid construction and luciferase assays Telomerase reverse transcriptase (TERT) promoter region was isolated from human genomic DNA using PCR, and the PCR products were cut with KpnI and XhoI or XhoI and HindIII restriction enzymes (New England Biolabs, Ipswich, MA), gel purified, and subcloned into pGL3-Enhancer Vector or pGL3Promoter Vector (Promega, Madison, WI). Article Title: Composition for regulating expression of PROX1 or expression of telomerase reverse transcriptase comprising activity regulator or method for screening telomerase reverse transcriptase regulator Article Snippet: The PCR product was digested with SacI and XhoI, and the prepared amplicons 1-3 were inserted into a Article Title: Egr-1 regulates the transcription of NGX6 gene through a Sp1/Egr-1 overlapping site in the promoter Article Snippet: These deletion fragments were cleaved, gel-purified and cloned into Activity Assay:Article Title: SINE Insertion in the Pig Carbonic Anhydrase 5B (CA5B) Gene Is Associated with Changes in Gene Expression and Phenotypic Variation. Article Snippet: These fragments were each inserted into the Article Title: NGX6 gene mediated by promoter methylation as a potential molecular marker in colorectal cancer Article Snippet: In order to identify the proximal promoter region of NGX6 gene, four constructs of progressive deletion were generated and cloned to the upstream of the luciferase reporter gene in the Article Title: Promoter Hypermethylation-mediated Inactivation of LRRC4 in Gliomas Article Snippet: The 5' upsteam regulatory region of the LRRC4 gene was subcloned into the KpnI and Bgl II restriction sites of the Article Title: Regulation of Cysteinyl Leukotriene Receptor 2 Expression—A Potential Anti-Tumor Mechanism Article Snippet: Human CysLT 2 R promoter fragments were subcloned into a Article Title: A Genetic Variant in the IL-17 Promoter Is Functionally Associated with Acute Graft-Versus-Host Disease after Unrelated Bone Marrow Transplantation Article Snippet: The resultant fragments IL-17/197A or IL-17/197G were subcloned into the pGL3-enhancer vector at the MluI and Article Title: Genetic Variants in Telomerase Reverse Transcriptase Contribute to Solar Lentigines. Article Snippet: Plasmid construction and luciferase assays Telomerase reverse transcriptase (TERT) promoter region was isolated from human genomic DNA using PCR, and the PCR products were cut with KpnI and XhoI or XhoI and HindIII restriction enzymes (New England Biolabs, Ipswich, MA), gel purified, and subcloned into pGL3-Enhancer Vector or pGL3Promoter Vector (Promega, Madison, WI). Article Title: Composition for regulating expression of PROX1 or expression of telomerase reverse transcriptase comprising activity regulator or method for screening telomerase reverse transcriptase regulator Article Snippet: The PCR product was digested with SacI and XhoI, and the prepared amplicons 1-3 were inserted into a Article Title: Egr-1 regulates the transcription of NGX6 gene through a Sp1/Egr-1 overlapping site in the promoter Article Snippet: These deletion fragments were cleaved, gel-purified and cloned into Generated:Article Title: SINE Insertion in the Pig Carbonic Anhydrase 5B (CA5B) Gene Is Associated with Changes in Gene Expression and Phenotypic Variation. Article Snippet: These fragments were each inserted into the Article Title: NGX6 gene mediated by promoter methylation as a potential molecular marker in colorectal cancer Article Snippet: In order to identify the proximal promoter region of NGX6 gene, four constructs of progressive deletion were generated and cloned to the upstream of the luciferase reporter gene in the Article Title: Promoter Hypermethylation-mediated Inactivation of LRRC4 in Gliomas Article Snippet: The 5' upsteam regulatory region of the LRRC4 gene was subcloned into the KpnI and Bgl II restriction sites of the Article Title: Regulation of Cysteinyl Leukotriene Receptor 2 Expression—A Potential Anti-Tumor Mechanism Article Snippet: Human CysLT 2 R promoter fragments were subcloned into a Article Title: A Genetic Variant in the IL-17 Promoter Is Functionally Associated with Acute Graft-Versus-Host Disease after Unrelated Bone Marrow Transplantation Article Snippet: The resultant fragments IL-17/197A or IL-17/197G were subcloned into the pGL3-enhancer vector at the MluI and Article Title: Genetic Variants in Telomerase Reverse Transcriptase Contribute to Solar Lentigines. Article Snippet: Plasmid construction and luciferase assays Telomerase reverse transcriptase (TERT) promoter region was isolated from human genomic DNA using PCR, and the PCR products were cut with KpnI and XhoI or XhoI and HindIII restriction enzymes (New England Biolabs, Ipswich, MA), gel purified, and subcloned into pGL3-Enhancer Vector or pGL3Promoter Vector (Promega, Madison, WI). Article Title: Composition for regulating expression of PROX1 or expression of telomerase reverse transcriptase comprising activity regulator or method for screening telomerase reverse transcriptase regulator Article Snippet: The PCR product was digested with SacI and XhoI, and the prepared amplicons 1-3 were inserted into a Article Title: Egr-1 regulates the transcription of NGX6 gene through a Sp1/Egr-1 overlapping site in the promoter Article Snippet: These deletion fragments were cleaved, gel-purified and cloned into |
